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Fig. 1. (A) TIRF-images of a cell transfected with P2X2-mRFP1 (left panel) and IAPP-EGFP (right panel). (B) Average image of 23 small frames taken from images like those shown in A and centered on the location of granules (right panel, IAPP-EGFP spots). The left panel is the average image of similar frames cut at the same locations from the red channel. Note uniform P2X2-mRFP1 labeling (left). (C) Sequence of confocal images showing Fluo-5F fluorescence in a cell that expressed P2X2-mRFP. At t=0 seconds, ATP (2 mM) was applied to the cell via a puffer pipette from the direction indicated with an arrow.





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