spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 7. PI 3-kinase activation by E4-ORF1 is not required to block TJ localization of PDZ proteins in MDCK cells. (A) Endogenous ZO-1 fails to associate with TJs in MDCK cells expressing mutant IIB E4-ORF1. Cells of the MDCK-IIB pool were immunostained with ZO-1 (middle panel) or E4-ORF1 (left panel) antibodies. The combined images are shown in the right panel (merge). Scale bars: 40 µm. (B) Endogenous PATJ and ZO-2 also fail to associate with TJs in MDCK cells expressing mutant IIB E4-ORF1. Cells of the MDCK-IIB pool were immunostained with ZO-1, PATJ, and ZO-2 antibodies. The top three panels represent the same field of cells showing immunostaining of ZO-1, PATJ, or the merge of the latter two images (merge). The bottom three panels represent the same field of cells showing immunostaining of ZO-1, ZO-2, and the merged images (merge). Dashed lines outline groups of cells inferred to express E4-ORF1 based on the results shown in (A).





Right arrow Return to article