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Fig. 14. Failure of complementation of Lim2 with paxB. To asses whether PaxB functions partly redundantly with its closest homologue Lim2 we performed synergy experiments between lim2– and cells of a strain overexpressing a PaxB-GFP fusion protein under the control of the strong actin15 promoter in the lim2– background. After 18 and 36 hours the cells are still stuck in the mound stage and the distribution of the lim2– cells overexpressing PaxB-GFP is random within the mound, showing that over expression of PaxB cannot rescue the lim2– phenotype. When we tested sorting between a lim2–/paxB– and lim2– strain expressing PaxB-GFP we also found no evidence for further development and cell sorting after 18 and 36 hours of development, suggesting that lim2 and paxillin may act in the same process, with PaxB possibly upstream of Lim2. In both experiments the PaxB-GFP expressing cells were 10% of total cells.
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