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Fig. 4. PaxB-GFP foci co-localise with cell-substrate contact areas. Brightfield, interference reflection contrast and fluorescence images were recorded simultaneously on a confocal microscope. Dark areas in the reflection interference contrast image surrounded by brighter interference patterns correspond to parts of the cell in close proximity to the substratum, the small very dark structures in the adhesion area are part of the contractile vacuole network. As the cell extends a pseudopod that makes contact with the substratum (15 second timepoint) PaxB-GFP localises to contact sites in this new adhesion area. In the merged fluorescence and interference contrast images at the bottom, PaxB-GFP appears purple and the contact area in dark green. Scale bar, 10 µm. See also supplementary material, Movie 4 for dynamics of paxillin in relation to the contact area in vegetative stage cells.
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