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Fig. 5. Co-localisation of PaxB-GFP and ABD-mRFPmars. Confocal time series of vegetative wild-type Ax2 cell expressing PaxB-GFP and ABD-mRFPmars. Time is indicated in seconds. (Left panel) PaxB-GFP (green) localises to long lived stationary contact sites at the cell/substratum interface as indicated by the arrows that mark the same contact sites at different time points. (Centre panel) ABD-mRFPmars (red) accumulates at very short-lived contact sites as indicated by the arrows. (Right panel) Merged image showing that PaxB-GFP and ABD-mRFPmars localise to different contact sites. Scale bar, 10 µm. See also supplementary material, Movie 5 for dynamics of mRFPactin and PaxB-GFP in vegetative stage cells.





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