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Fig. 7. Characterisation of paxB knockout phenotypes. Comparison of Ax2 and an Ax2 paxB knockout strain (A,C) Ax2 and paxB in top view after 24 hours of development on agar. In Ax2 all cells develop to fruiting bodies, while in the paxB strains many cells do not develop into fruiting bodies but arrest at the mound stage, or when they continue development later development is grossly abnormal. (B,D) Ax2 and paxB knockout strain in side view after development on water agar for 24 hours. Note the differences in scale between images in B and D. Ax2 form fruiting bodies with slender stalks and well define basal disks while the paxB mutant often form small fruiting bodies on a large mass of cells that is not lifted off the substrate.





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