Depletion of GAK/auxilin 2 inhibits receptor-mediated endocytosis and recruitment of both clathrin and clathrin adaptors
J Cell Sci Lee et al.
118: 4311
JCS02548 Supplementary Material
Files in this Data Supplement:
Supplemental Figure 1
-
Fig.S1. Effect of GAK
depletion on BSA uptake, BFA sensitivity and EGFR expression. (A) Fluid phase
uptake in mock-depleted (a) and GAK-depleted cells (b). Cells were imaged using
the confocal microscope after internalizing Alexa546-BSA for 30 minutes at
37°C. (B) Confocal images of cytosolic GFP-clathrin in the identical
GAK-depleted cell were taken before (a) and after (b) BFA addition. (C) Western
blot showing the level of EGFR in mock-depleted (lane 1) and GAK-depleted cells
(lane 2). EGFR was immunoprecipated and then run on SDS gels and blotted as
described in Materials and Methods.
