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Fig. 9. Localization of clathrin adaptors on the plasma membrane and TGN is altered in GAK-depleted cells. (A) TIR-FM images of mock-depleted (a,c) and GAK-depleted (b,d) cells cotransfected with DsRed-clathrin (a,b) and GFP-AP2 (b,d). (B) TIR-FM images of mock-depleted (a,c) and GAK-depleted (b,d) cells cotransfected with DsRed-clathrin (a,c) and GFP-epsin (b,d). In the inset, the brightness was increased to better visualize the pits of the dimmer cell (boxed region) that was expressing clathrin and epsin at lower levels than the neighboring cell. (C) Confocal images of immunostained TGN-associated clathrin adaptors, AP1 (a,b) and GGA3 (c,d) in mock-depleted (a,c) and GAK-depleted (b,d) cells.





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