JCS02550 Supplementary Material

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• Supplemental Figure 1 - Fig.S1. Examples of isotype-specific labeling of b tubulin in the developing organ of Corti. Antibodies against b_II (D,E) and b_III (C) tubulin were applied to label whole mounts of developing rat organ of Corti using standard methods (Jensen-Smith, 2003a). (A) b_I tubulin stain at embryonic day 20 (E20), (B) b_I tubulin at E20, (C) b_III tubulin at E20, (D) b_II tubulin at postnatal day 1 (P1) (E) b_II tubulin at P1, (F) b_IV tubulin at P1. Scale bars 20 mm and *50 mm. Note the primary cilia on all epithelial cell types (especially apparent in A and D, indicated by large white arrowheads). IHCs, inner hair cells; OHCs, outer hair cells; OP, outer pillar cells; Deiter’s, Deiter’s cells (Vent et al., 2003). For more details, see (Hallworth and Luduena, 2000; Hallworth et al., 2000; Jensen-Smith et al., 2003a; Vent et al., 2003).

• Supplemental Figure 2 - Fig.S2. Preincubation experiment with antibody against b_I tubulin (the same results were obtained for anti b_IV and b_V tubulin).