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Fig. 1. (A) Effect of isotype-specific antibodies against ß tubulin on CBF. Final antibody concentrations 0.33 µM, error bars indicate 1 s.e.m. Filled black squares, control; open pink circle, anti ß_I tubulin; open upward blue triangle, anti ß_II tubulin; open downward navy triangle, anti ß_III tubulin; open red diamond, anti ß_IV tubulin; open purple triangle, anti ß_V tubulin. The antibodies depicted in shades of red blocked ciliary beating, the antibodies in blue had no effect on CBF. (B) Concentration dependence of anti ß_I tubulin; error bars indicate 1 s.e.m. Antibody concentrations: blue triangle, 0.33 µM; green triangle, 0.033 µM; red circle, 3.3 nM. Similar concentration curves were obtained for anti ß_IV tubulin and anti ß_V tubulin. (C) Effect of non-isotype-specific, non-C-terminal antibodies against and ß tubulin (DM1A, red circle and TUB 2.1, green triangle), error bars indicate 1 s.e.m.; antibody concentrations 0.33 µM. (D) Effect of anti tubulin C-(red circle) and N-terminal (green triangle), non-isotype-specific antibodies, error bars indicate 1 s.e.m.; antibody concentrations 0.33 µM.

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