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Fig. 2. CD45⁺ cells residing in the bone marrow and skeletal muscle are intrinsically distinct. Whole BM MNCs (white bars) and skeletal muscle MNCs (black bars) were isolated from GFP mice by collagenase digestion, and CD45⁺ cells were purified by FACS and (A) analyzed for the (i) frequency and (ii) type of CFU progenitors, and (iii) the frequency of B-cell progenitors. The number of experiments completed (n) is given with each graph. (B) CD45⁺ cells purified from BM and skeletal muscle were cultured for 2, 3, 4 and 5 weeks on confluent monolayers of MS-5 stroma. (i) At each time point the number of CD45⁺ cells arising from BM-derived () or skeletal muscle-derived () co-cultures was determined by flow-cytometry. (ii) The absolute number of Sca-1⁺CD45⁺ cells initially seeded at day 0 and following 2 weeks in culture was also examined and the fold-expansion of (iii) Sca-1⁺CD45⁺ cells and (iv) CFUs evaluated in 2-week and 5-week cultures (n=5-8). ^* indicate statistically significant differences compared with skeletal muscle, with P<0.05.

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