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Fig. 3. BM-derived CD45⁺ cells and progenitors are capable of migrating towards mature muscle cells. BM-MNCs (500,000 per well) derived from GFP mice were loaded into the upper chamber of a transwell apparatus that contained a monolayer of MS-5 stromal cells, C2C12-derived myotubes, primary skeletal muscle cells derived from adult CD-1 mice, or random migration-controls consisting of stroma culture media or myogenic culture media alone in the lower chamber as indicated. After 24 hours, cells were harvested from the lower chambers and the number of CD45⁺ cells and CFU progenitors was determined by flow-cytometry and methylcellulose assays, respectively. Statistically significant differences from random migration controls are indicated by an ^* (P0.05; n=11, or n=3 when primary muscle cells were used). BFU-E, CFU-M, CFU-G, CFU-GM, CFU-GEMM are defined as Burst-forming-unit erythroid, colony-forming-unit macrophage, colony-forming-unit granulocyte, colony-forming-unit granulocyte-macrophage and colony-forming-unit granulocyte-erythroid-macrophage-megakaryocyte, respectively.

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