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Fig. 4. Myofiber and stroma-migrating CD45⁺ cells and CFUs represent distinct populations of bone marrow hematopoietic cells. (A) Schematic diagram of the procedure used for analysis of the 2° migration potential of migrating and non-migrating cells isolated from 1° myofiber migration assays. (B) Twenty-four hours after loading 1° migrating (i) and non-migrating (ii) cells (100,000 to 500,000 cells per well) into 2° migration assays, the number of CD45⁺ cells and CFU progenitors migrating to confluent layers of C2C12-derived myotubes (red), MS-5 stroma (blue) or culture media alone was determined. ^* indicate statistically significant differences in the percentage of migrating cells compared with 2° migration-controls containing stromal cell (white) or myofiber (gray) growth media in the lower chambers (P $≤$ 0.05).

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