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Fig. 5. Effect of knocking down CRK1 and CRK2 on the numbers of nuclei and kinetoplasts and BrdU incorporation in bloodstream-form cells. (A) 3 days after RNAi induction, cells were stained with propidium iodide and examined with a fluorescence microscope for tabulation of cells with different numbers of nuclei (N) and kinetoplasts (K). Data are presented as the mean percentage (±s.e.) of total cells counted (>200) from three independent experiments. (B) BrdU incorporation analysis. The double knockdown cells were labeled with BrdU 3-5 days after the initiation of RNAi and examined by immunofluorescence assay.





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