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Fig. 6. Effects from double knockdowns of CRK1 and CRK2 on the morphology of bloodstream-form cells. (A) Cells 3 days after RNAi induction were stained with propidium iodide and examined with a fluorescence microscope. Upper panel, 1N1K, 1N2K and 2N2K control cells without RNAi induction. Lower panel, CRK1+CRK2-depleted 1N1K, 1N2K and 2N2K cells. (B) A CRK1+CRK2-depleted cell stained with YL1/2 antibody, showing the absence of newly synthesized microtubule from the posterior end.