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Fig. 1. Cellular distribution of IRP1 and IRP2. (A,B) Distribution of (A) IRP1 using an IRP1 antibody (red) and (B) IRP2 using an IRP2 antibody (red), in NIH3T3 cells. The cell nuclei are visualized with DAPI (blue). Both A and B reveal a cytosolic distribution for IRP1 and IRP2 but IRP2 distribution is more punctate than IRP1. (C,D) Distribution of (C) c-myc-tagged IRP1 and (D) c-myc-tagged IRP2, in NIH3T3 cells. C and D are representative cells that show the cytosolic distribution of both IRP1 and IRP2 (clear area is the cell nucleus). IRP1 is localized more in the perinuclear region whereas IRP2 localization extends further toward the perimeter of the cell.





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