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Fig. 9. Electrophoretic mobility shift assays (EMSAs) for IRPs in SW1088 cells in response to phorbol 12-myristate 13-acetate (PMA, 0.2 µM) and chelerythrine (CET, 2.0 µM). IRP/IRE interaction in SW1088 cells decreases upon treatment with PMA in both the cytosolic and membrane fractions and increases upon treatment with CET in both the cytosolic and membrane fractions. The data suggest IRP localization to the cytosolic fraction occurs with protein kinase C inhibition. *P<0.05.





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