QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 1. Western blots of parietal cell cultures probed for ezrin, CFP-ezrin construct expression, and for H,K-ATPase. Conditions for cell culture included control cells (C, no treatment), control virus infected cells (CV), and cells infected with rAD including CFP-constructs as wild-type ezrin (WT), T567A mutant ezrin (TA), and T567D mutant ezrin (TD). (A) Blot probed with an antibody against ezrin that recognizes 80 kDa native ezrin, the CFP-constructs of ezrin (106 kDa) and an N-terminal 55 kDa breakdown product of ezrin. (B) Blot probed with antibody against GFP which also recognizes CFP. For blots A and B, protein bands are marked by arrowheads indicating lanes for ezrin (ezrin), the CFP-tagged ezrin constructs (CFP-ez), the approximately 55 kDa ezrin breakdown product (55 kD), and CFP alone (CFP) in the cells infected with the control rAd construct. (C) Blot probed with antibody against the ß-subunit of H,K-ATPase (HKß) to show the approximate equivalency of parietal cells in the assay. The antibody against the ß-subunit of H,K-ATPase ordinarily migrates as a very broad band, 60-80 kDa, because of the high degree of glycosylation.

Return to article