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Fig. 4. Time course of stimulatory response for cells infected with rAD-CFP-ezrin T5687D mutant. Cells were placed in an incubating chamber (37°C) and viewed by spinning disc confocal microcopy using the CFP channel. Histamine plus IBMX was added at zero time and the cells were periodically examined with images being selected at the times (in minutes) shown. For the two parietal cells expressing CFP-ezrin T567D in the main panel the CFP signal was at the basolateral surface in the form of long surface projections; there was no significant morphological response to stimulation. The rectangular inset at the lower left has digitally amplified an extremely faint image (virtually autofluorescence) of that very region and the signal to show that `hidden' parietal cells on the same slide, but not expressing T567D, respond normally to stimulation. Bar, 20 µm for all images.
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