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Fig. 7. Scanning electron microscopy of parietal cell cultures in control conditions and those infected with rAd-CFP-ezrin constructs expressing WT and the T567A and T567D mutants. The cells were cultured on silicon wafers, exposed to virus (or not) for 48 hours, then fixed, dried, coated with carbon and examined in a Hitachi scanning microscope as described in Materials and Methods. For most of the cells/treatments the plasma membrane (basolateral membrane) is studded with short projections and folds, with occasional lamellipodia and longer microvillar projections. In the case of T567D the basolateral membrane was often found to be replete with extensive long microvillar projections. Bars, 5 µm.
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