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Fig. 1. Cannabinoid and vanilloid receptors in sperm cells. (A) Saturation curves of [3H]CP55.940 binding to mouse brain, mouse spleen or boar sperm membranes. (B) Effect of CB1 and CB2 receptor antagonists SR141716 and SR144528, and of natural (AEA) or synthetic (Met-AEA) agonists on the binding of 400 pM [3H]CP55.940 (100% as in A). *P<0.01 versus control (CTR). (C) Saturation curves of [3H]RTX binding to boar sperm membranes, alone or in the presence of 1 µM CPZ. The effect of 1 µM AEA (#) or 1 µM Met-AEA (@) on the binding of 1600 pM [3H]RTX is also shown. In all panels, vertical bars represent the s.d.
