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Fig. 2. Expression of CB1, CB2 and TRPV1 receptors, and FAAH, in sperm cells. (A) Western blot analysis of sperm cell extracts (20 µg/lane) reacted with specific polyclonal antibodies. Gels were overstained in order to check for antibody specificity. Molecular mass markers are shown on the right side. (B) ELISA test of resting or capacitated sperm cells, reacted with the same antibodies as in A. Values were expressed as percentage of the resting cells, set to 100 (100%=0.340±0.040, 0.270±0.030, or 0.300±0.030 absorbance units at 405 nm for CB1R, TRPV1 or FAAH, respectively). In B, horizontal bars represent the s.d.; *P<0.05, **P<0.01 versus resting sperm cells.





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