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Fig. 7. Role of AMT and TRPV1 in acrosome reaction (AR). Spontaneous or ZP-induced AR recorded in spermatozoa capacitated in vitro for 4 hours in the absence (CTR) or presence of 1 µM Met-AEA, 1 µM CPZ, 10 µM VDM11 or their combinations. Standard deviation values ranged amongst experiments from 0.8 to 3.6. Statistical analysis of spontaneous AR data revealed the following: P<0.01 for CTR versus CPZ, CPZ versus CPZ plus VDM11, and CPZ versus CPZ plus VDM11 plus Met-AEA; P<0.05 for CTR versus Met-AEA plus CPZ, and CPZ versus Met-AEA plus CPZ. Statistical analysis of ZP-induced AR revealed the following: P<0.01 for CTR versus Met-AEA, CTR versus CPZ, CTR versus Met-AEA plus CPZ, CTR versus Met-AEA plus VDM11, and CTR versus Met-AEA plus CPZ plus VDM11.





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