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Fig. 1. Expression of FAK/YCam and Y397F-FAK/YCam in U87 astrocytoma cells. (A) Schematic representation of FAK with the phospho-acceptor residues and the epitopes recognized by the different antibodies used in this study. (B) Control U87, FAK/YCam (FAK) and Y397F-FAK/YCam (Y397) cells were lysed and immunoblotted for FAK with antibodies that recognize the kinase domain (top panel), the C-terminal domain (Ct; middle panel) or the FAT domain (lower panel). The lower bands correspond to endogenous FAK (125 kDa), while the upper bands correspond to the fusion proteins FAK/YCam or Y397F-FAK/YCam (199 kDa).