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Fig. 5. Effects of mutation at Tyr-397 on the dynamics of FAK/YCam after nocodazole washout observed by confocal imaging during 1 hour of cells transfected with FAK/YCam (A), Y397F-FAK/YCam (B) and non transfected cells (C). White arrows show positions of FAK/YCam-containing FAs that were highly dynamic with clear cell edge movements (A). White arrow heads indicate positions of Y397F-FAK/YCam-containing FAs that were stable (B). A non transfected cell adjacent to the cell transfected with Y397F-FAK/YCam displays normal motility, with cell edge retraction (grey arrow, C). Images are representative of cells from a minimum of for separate experiments. Scale bar: 5 µm.





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