spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 1. Three-dimensionality of cell-free 3D fibronectin matrix. 3D matrix prepared from WI-38 (VA13) cells (A) and a monolayer of WI-38(VA13) cells (B) were immunostained with anti-human fibronectin antibody HFN7.1 and analyzed by confocal microscopy. 0° is the projected image and 90° the 3D image rotated through 90 degrees to show thickness. The basal cell surfaces are to the left. (C) Equivalent proportions of NIH3T3 3D matrix (lane 1) and confluent cell culture (lane 2) solubilized with SDS sample buffer were compared by SDS-PAGE using 6% polyacrylamide and silver staining. The major bands in lanes 1 and 2 were confirmed as fibronectin by immunoblotting (not shown). The location of fibronectin at 250 kDa and molecular mass markers (180 kDa and 116 kDa) are indicated.





Right arrow Return to article