spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. Increased fibronectin matrix assembly by WI-38 (VA13) cells on a 3D fibronectin matrix. WI-38(VA13) cells were seeded on 3D matrix prepared from NIH3T3 cells (3D) or on surfaces coated with 10 µg/ml rat pFN (2D). (A) DOC lysates were prepared at the indicated times. DOC-insoluble samples were analyzed on 5% polyacrylamide-SDS gels and immunoblotted with HFN7.1. (B) Cells were seeded in 24-well dishes at the indicated cell densities. After incubation for 6 hours, DOC-insoluble samples were prepared and analyzed as described in A. (C) WI-38 (VA13) cells seeded for 8 hours on 3D matrix (3D, left) or fibronectin-coated coverslips (2D, right) were fixed and immunostained with HFN7.1 followed by fluorescein goat anti-mouse IgG. (D) DOC-insoluble fibronectin matrix formed by WI-38 human lung fibroblasts (left) and human dermal fibroblasts (right) was analyzed at 8 hours as in A. Bar, 20 µm.





Right arrow Return to article