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Fig. 5. Distribution of newly assembled and pre-existing fibrils formed on a 3D matrix. CHO(B2){alpha}5 cells were incubated in medium with 4 µg/ml rat pFN on coverslips with 3D human fibronectin matrix. After incubation for 15 hours, samples were fixed and stained sequentially with IC3 and fluorescein-goat anti-mouse IgG to detect rat fibronectin fibrils followed by biotinylated HFN7.1 and rhodamine-streptavidin to detect human fibronectin 3D matrix. (A) Projected confocal images of newly assembled rat pFN fibrils (left), 3D human fibronectin matrix (middle) and the merged image (right). (B) Fluorescence intensity profiles of four areas (lines 1-4 in merged image of A) were analyzed using laser-scanning microscopy LSM 510 version 3.2 software (Zeiss).





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