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Fig. 7. Controls for the effects of 3D matrix pliability. (A) CHO(B2){alpha}5 cells were plated in medium supplemented with rat fibronectin on Matrigel (MG), Matrigel containing 60 µg/ml fibronectin (MG+FN), 3D fibronectin matrix (3D) or 2D fibronectin substrate (2D). (B) Cells were plated in medium supplemented with rat fibronectin (FN) on 3D fibronectin matrix (3D), 2D fibronectin substrate (2D), or 3D fibronectin matrix that had been fixed in 4% paraformaldehyde (fixed 3D). 3D and fixed 3D (right lanes) were incubated with fibronectin but without cells. (C) Cells were seeded on 3D substrates in the absence (-) and presence (+) of 100 µg/ml 9D2 antibody for 7 hours. DOC-insoluble fractions were analyzed by immunoblotting with IC3.





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