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Fig. 10. UIM-dependent localization of Eps15 into ubiquilin-containing cytoplasmic aggregates. HeLa cells transiently co-transfected with the GFP-ubiquilin construct and with the FLAG-Eps15 (a,b) or the FLAG-Eps15-{Delta}UIM1+2 (c,d) construct were fixed, permeabilized and processed for fluorescence microscopy using the anti-FLAG antibody followed by Cy3-labelled goat anti-mouse immunoglobulin secondary antibodies. (a,c) Green fluorescence emitted by GFP. (b,d) Red fluorescence emitted by Cy3. Insets show higher magnifications of representative areas.





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