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Fig. 1. BICR-M1Rk cells assemble untagged and fluorescent-protein-tagged Cx26 and Cx43 into gap junctions via a trafficking pathway that is BFA-sensitive. Wild-type BICR-M1Rk (A,B) or BICR-M1Rk stably expressing Cx43-GFP (C,D), Cx26 (E,F) or Cx26-YFP (G,H) were immunolabeled for either Cx43 (A,B) or Cx26 (E,F); fluorescent-protein-tagged connexins were imaged without further processing. There was a prominent untagged or fluorescent-protein-tagged paranuclear-localized and plasma membrane-localized connexin population in untreated cells (A,C,E,G). However, following BFA treatment, all connexin variants were localized in an ER-like pattern (B,D,F,H) and gap junction plaques were not evident. Bar, 10 µm. (I) BFA-treatment of cells co-expressing Cx43 and Cx26 (BICR Cx26) dramatically inhibited dye coupling. Data expressed as mean number of cells receiving dye before or after BFA treatment±s.e.m.





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