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Fig. 2. Expression of mutant Sar1 disrupts ER-to-Golgi transport in BICR-M1Rk cells and inhibits Cx26 and Cx43 trafficking to the cell surface. BICR-M1Rk cells were transiently transfected with either Sar1-DsRed2 (A-C) or mutSar1-DsRed2 (D-F), and immunolabeled for the Golgi-resident protein GPP130 (A,D). The Golgi apparatus, as defined by GPP130 localization (arrows), remained intact in the presence of Sar1-DsRed2 but was disrupted in cells expressing mutSar1-DsRed2. BICR-M1Rk cells stably expressing Cx26-YFP were transiently transfected with Sar1-DsRed2 (G-J) or mutSar1-DsRed2 (K-N) and immunolabeled for Cx43. Anti-rabbit AMCA was used to visualize and separate the Cx43 signal from YFP and DsRed fluorescence. Sar1-DsRed2 did not disrupt the localization pattern of Cx43 or Cx26-YFP but mutSar1-DsRed2 caused their retention within the ER. Bars, 10 µm. WT, wild-type; MUT, mutant.





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