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Fig. 9. Steady-state regeneration of Cx43-GFP gap junctions is sensitive to BFA and nocodazole. NRK cells stably expressing Cx43-GFP were time-lapse imaged following photobleaching of a series of gap junctions in the presence of BFA or nocodazole. Control cells revealed substantial recruitment of Cx43-GFP to the photobleached gap junctions after 1 hour (A,D,G) but this level of recovery was not observed in cells treated with BFA (B,E,H) or nocodazole (C,F,I). Panel inserts represent higher magnifications of the photobleached regions of interest. Experimental sets were repeated six times with similar results. Bar, 10 µm.





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