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Fig. 4. Ca²⁺ mobilization activates PLC in primary mouse pancreatic ß-cells. (A) Mouse pancreatic ß-cells transiently transfected with PH_PLC-YFP and loaded with Fura Red were transferred to medium containing 20 mM glucose, 250 µM diazoxide, 50 µM verapamil and 100 nM glucagon. PLC activity (PH_PLC-YFP fluorescence, green trace) and [Ca²⁺]_i response (red trace) were recorded simultaneously with an image pair every 0.7 seconds. The Fura Red trace has been inverted to show [Ca²⁺]_i increases as upward deflections. (B) Time expansions of the shaded regions in A showing that the [Ca²⁺]_i responses precede PLC activation. The recording is representative for six cells from five independent experiments.

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