|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 5. PKC activation increases neurite outgrowth in SGN cultures. (A) Representative photomicrographs of TUJ1-stained SGNs in 24 hour culture treated without (CTR) or with 100 nM PMA (PMA). Arrows in the control condition show aneuritic neurons usually found in 24 hour control cultures. Bar, 100 µm. (B) SGNs were treated for 24 hours with or without increasing concentrations of PMA and in combination or not with the specific PKC inhibitor, i.e. GF (1 µM). Subsequently, cells were fixed and immunostained with TUJ1 antibody and the neuritic index in each condition as a percentage of the neuritic index in the untreated control condition was determined (n=6; ***P<0.001).
|
