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Fig. 6. The survival-promoting action of neurotrophins in SGNs depends on PI3K and MEK/ERK signalling but is independent of PKC activity. (A) SGNs were cultured in the presence of BDNF or NT3 (each at 20 ng/ml) plus LY294002 or U0126 (each at 20 µM). Cells were fixed 24 hours later and the remaining number of viable neurons were counted, as determined in Fig. 2A (n=6; ***P<0.001). (B) SGNs were treated with 100 nM PMA, NT3 or BDNF (each at 20 ng/ml) in the presence or absence of 1 µM GF109203X to block the enzymatic activity of PKC. 24 hours later, cells were fixed and neuronal survival was scored, as described in Fig. 2A (n=9; ns, not significant; ***P<0.001).
