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Fig. 7. PKC activation and neurotrophins synergistically enhance survival of SGNs. SGNs were cultured for 96 hours in the presence of different combinations of PMA (P, 100 nM), BDNF (B, 100 ng/ml) and NT3 (N, 100 ng/ml). Cells were fixed and neuronal viability in the different experimental conditions was expressed as a percentage of the average number of TUJ1-positive neurons in PMA condition. Black bars represent measurements observed in experimental conditions and grey bars represent theoretical values calculated by considering the experimental survival effect of each factor alone (n=15; ns, not significant; ***P<0.001).





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