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Fig. 7. Endogenous SNX2 shows a high degree of co-localisation with the retromer component mVps26. (A) Co-localisation of endogenous SNX2 and endogenous mVps26. Cells were plated on coverslips, fixed and stained for SNX2 and mVps26. (B) Suppression of SNX1, SNX2, or joint suppression of both SNX1 and SNX2 does not affect mVps26 localisation. HeLa cells were treated with control, SNX1-specific siRNA, SNX2-specific siRNA or jointly with SNX1- and SNX2-specific siRNA duplexes for 72 hours. Cells were then fixed and stained against endogenous SNX1, endogenous SNX2 and endogenous mVps26. Individual or joint suppression of SNX1 and SNX2 does not affect punctate distribution of mVps26. Bars, 20 µm (A,B).





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