spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 3. (A) Northern analysis of SRP72, SRP68 and SRP19 mRNA upon silencing. RNA was prepared from cells carrying the RNAi construct, uninduced (-Tet) and after 2-3 days of induction (+Tet). Total RNA (20 µg) was subjected to northern analysis with random-labeled probes. (1) SRP72-depleted cells. (2) SRP68-depleted cells. (3) SRP19-depleted cells. The transcripts of SRP72, SRP68, SRP19, tubulin mRNAs, rRNA and dsRNA are indicated by arrows. (B) Western analysis of SRP19 protein. Whole cell extract (106 per lane) was prepared from cells carrying the RNAi construct, uninduced (-Tet) and after 4 days of induction (+Tet). The extract was fractioned on a 12% SDS-polyacrylamide gel and subjected to western analysis with the anti-SRP19 antibodies. SRP19 and a non-specific protein band used as a control for the amount of protein loaded are indicated by arrows.





Right arrow Return to article