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Fig. 5. Mitotic transients are dependent on extracellular Ca²⁺. Pronucleate embryos were transferred to Ca²⁺-free medium containing 1 mM EGTA 28-29 hours after hCG administration, at which time $~$ 10% had undergone NEBD. (A) No change in the Fura ratio was detected at NEBD or during mitosis in Ca²⁺-free medium in any case (n=14). (B) Transfer to Ca²⁺-free medium had no effect on the timing of NEBD and cytokinesis (data from two similar replicates; n=46 for Ca²⁺-containing medium, n=51 for Ca²⁺-free medium). (C) Hoechst labelling revealed chromatin within the nucleus of each resulting blastomere, indicating that chromosome disjunction is not dependent on extracellular Ca²⁺ (Ca²⁺-free medium, n=13; control, n=6). Open arrow, NEBD; closed arrow, cytokinesis.

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