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Fig. 7. The nuclear increase in indicator fluorescence occurs concomitantly with nuclear-membrane permeabilization. Parthenogenetic embryos were co-injected with Ca2+-Green/dextran and a 70 kDa rhodamine-dextran. The 70 kDa rhodamine-dextran was excluded from the nucleus, allowing NEBD to be determined accurately as the time at which the rhodamine signal entered the nucleoplasm (Aa'-d'). The changes in fluorescence of the two indicators were examined using regions of interest placed in the cytoplasm and nucleus (Bi). Lower-case letters indicate which image corresponds to given points on the graph. Notice that the nuclear increase in Ca2+-Green fluorescence occurs at precisely the same time that the 70 kDa rhodamine-dextran enters the nucleoplasm (Bii).





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