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Fig. 2. Effect of CD87 and CD222 co-expression on TGF-ß activation. Mouse CD222-/- fibroblasts (A) stably transduced with CD222 (B), CD87 (C) or both CD222 and CD87 (D) were cultivated in 96-well plates (5000 cells per well) and treated with 20 µg ml-1 Plg or 1 µg ml-1 suPA for 12 hours. The interfering agents were used in following concentrations: aprotinin (apro), 2 µg ml-1; pepB or pepC, 20 µg ml-1. After incubation, the culture supernatant was analysed for the presence of active TGF-ß by the MLEC assay. Results are expressed as concentration of active TGF-ß in ng ml-1.





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