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Fig. 7. Analysis of CD222 and CD87 expression and complex formation in ECs versus SMCs. (A) FACS analysis using specific mAbs. Cell-surface expression of CD222, CD87 and CD147 on HUVECs and HUVSMCs was analysed by immunofluorescence analysis and flow cytometry using the anti-CD222 mAb MEM-238, the anti-CD87 mAb C8 and the anti-CD147 mAb MEM-M6/1. (B) Surface-biotinylated HUVECs and HUVSMCs were subjected to immunoprecipitation using MEM-238. The precipitate was immunoblotted with MEM-238, the anti-CD87mAb H2 or the control mAb AFP-01. (C) HUVECs and SMCs were grown in gelatin-coated coverslips, washed, fixed, blocked and stained with the specific fluorescently labeled antibodies MEM-238/AF555 (CD222, red) and E4-AF488 (CD87, green). The slides were analysed under a confocal laser-scanning microscope. Colocalization structures (yellow) are indicated by arrows. All images show a region of 110x110 µm.





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