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Fig. 5. The morphology of procyclic-form trypanosomes induced by ectopic overexpression of TbZFP2. (A) A phase contrast image of procyclic forms, one of which shows a greatly extended posterior end (nozzle). (B) A composite of the DNA of the trypanosome cell (labelled with DAPI and pseudocoloured blue; each cell contains a central nucleus and posterior kinetoplast) and staining with an antibody (YL1/2; Kilmartin et al., 1982) against tyrosinated {alpha}-tubulin, which labels dynamic microtubules in the trypanosome cytoskeleton (pseudocoloured green). Note that the posterior end of each cell shows staining, as does the newly growing daughter flagellum (labelled F) and the basal bodies (labelled in two of the cells with an asterisk). The nozzle cell shows an extended region of staining at the posterior end of the cell, indicating microtubule extension in this region. Bar, 8 µm.





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