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Fig. 3. F2101P/K2103P mutations in full-length CBP disrupt protein-protein interaction and colocalization with SRC-1. (A) Subcellular localization of YFP-CBP(F2101P/K2103P) (YFP-CBP/H3P). HEK 293 cells were transfected with 400 ng of pYFP-CBP/H3P and treated for 2 hours with 100 µM CoCl₂. Bar, 3 µm. (B) YFP-CBP/H3P shows diffuse intranuclear distribution and does not colocalize with SRC-1. HEK 293 cells were transfected with 400 ng of expression plasmids encoding CFP-SRC-1, CFP-HIF-1 ${alpha}$ and/or YFP-CBP/H3P as described in Fig. 1A. Bar, 3 µm. (C) YFP-CBP/H3P fails to coprecipitate with endogenous SRC-1 in HEK 293 cells. Cells were transfected with 1 µg of either pYFP-CBP or pYFP-CBP/H3P and kept at normoxia (N) or treated with 100 µM CoCl₂ (C) for 3 hours. Whole cell extracts corresponding to a total protein concentration of 3 mg were incubated with anti-SRC-1 antibody ( ${alpha}$ -SRC-1) for 2 hours at room temperature. Immunoprecipitated protein complexes were separated as before and immunoblotted using anti-GFP ( ${alpha}$ -GFP) and ${alpha}$ -SRC-1 antibodies. ^*Non-specific band. 50 µg of whole cell extract protein was loaded on input gels.

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