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Fig. 1. The effects of Wnt3a on activation of the Wnt/ß-catenin pathway. (A) NIH3T3 cells were grown to 70% confluence and either not treated or treated with 150 ng/ml of recombinant Wnt3a for 8 hours. Whole cell lysates were subjected to western blotting with ß-catenin, cyclin D1 or -tubulin primary antibodies. (B) NIH3T3 cells were grown to 50% confluence, then transfected with either 0.5 µg of pTOPFLASH or pFOPFLASH (Korinek et al., 1997). 48 hours after transfection, cells were harvested for a Luciferase assay. Where required, 150 ng/ml of recombinant Wnt3a was applied 8 hours before harvesting. The mean values and standard deviations of three independent experiments are shown.

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