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Fig. 2. Characterization of C. lindemuthianum strains transformed with PG2-90::CLPT1 or PG2-90::CLPT1(N123I) constructs. (A) Northern blot analysis of total RNA extracted from the different strains grown on pectin medium and probed with CLPT1 coding sequence. The band from the wild-type gene is indicated by the arrowhead. (B) Detection by western blot analysis of CLPT1 from wild-type and transgenic strains. Protein extracts were prepared from the mycelium grown for 4 days on glucose or pectin medium as indicated. Equal amounts of protein were loaded onto the gel. CLPT1 was revealed using purified anti-MBP-CLPT1 antibodies and anti-rabbit IgG labelled with alkaline phosphatase as secondary antibodies.





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