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Fig. 5. Effect of CLPT1(N123I) on ultrastructural morphology. Conidia from the wild-type strain or the transgenic strains were allowed to germinate on a cellophane membrane placed on top of a solid medium containing glucose. After 24 hours of growth, the filters were transferred to a fresh medium containing either glucose (A-C,G,H) or pectin (D-F,I,J) as sole carbon sources. Micrographs of ultrathin sections of hyphae stained with (A-F) uranyl acetate and lead citrate or (G-J) PATAg. Arrowheads indicate the vesicle-like material; white arrows, the large spots accumulated within the hyphae of the CLPT1 (N123I)-overexpressing strains grown on pectin. Bars, 1 µm.





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