QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 3. The proline-rich domain of p42^POP mediates profilin binding. (A) Yeast two-hybrid analysis with profilins 1 and 2a (PFN1/2a) as a bait and different p42^POP fragments as prey. Only fragments containing the proline clusters gave a positive reaction (+). (B) Autoradiograms obtained after SDS-PAGE of pellets (P) and supernatants (S) derived from pull-down assays with profilin-1-coupled Sepharose (PFN1) or BSA-coupled Sepharose (control). Both types of Sepharose bead were incubated with in-vitro-translated, radioactively labelled full-length p42^POP or the N-terminal (N-POP) or C-terminal (C-POP) fragment. Only C-POP was precipitated by PFN1 beads.

Return to article