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Fig. 7. p42^POP localizes with profilin 1 and recruits profilin 1 into the nucleus. Fluorescence images obtained as optical sections by confocal laser-scanning microscopy revealed that GFP-p42^POP (A) and profilin 1, seen by immunostaining with a monoclonal antibody (A'), are both localized to the nucleus, in a fine granular pattern with some overlap (A''). Bar, 10 µm. (B) Quantitation of nuclear profilin (PFN1) in PtK2 cells transiently transfected with GFP-fused p42^POP, POP4 or POP6. The bars display the relative fluorescences of the profilin-specific signals obtained for cells transfected with p42^POP, POP4 or POP6 compared with values obtained with untransfected cells on the same coverslip (defined as 1.0). Values were obtained for 150 cells and are shown with standard errors compared by variance analysis (Student-Newman-Keuls; P<0.05). Expression of p42^POP raised the nuclear profilin level by 17% in comparison with untransfected cells, whereas neither POP4 (which lacks the proline clusters) nor POP6 (which does not target to the nucleus) (cf. Fig. 2) showed this increase in nuclear profilin.

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